强化融合蛋白,ER(Fv-LDP-AE)
1)ER(Fv-LDP-AE)强化融合蛋白
1.Fusion protein ER(Fv-LDP) was prepared and energized fusion protein ER(Fv-LDP-AE) was reconstituted.利用基因重组技术构建表达单链抗体与力达霉素辅基蛋白的融合蛋白ER(Fv-LDP),并与力达霉素发色团组装成强化融合蛋白ER(Fv-LDP-AE),研究其对EGFR高表达肿瘤细胞的体外杀伤作用以及体内抑瘤活性。
英文短句/例句
1.Construction of Energized Fusion Protein Consisting of Anti-HER2 scFv and Lidamycin and Its Antitumor Activity;抗HER2单链抗体与力达霉素强化融合蛋白的构建及其抗肿瘤活性研究
2.Construction of energized fusion protein consisting of epidermal growth factor receptor oligopeptide ligand and lidamycin and its antitumor activity靶向表皮生长因子受体的寡肽与力达霉素强化融合蛋白的构建及其抗肿瘤活性
3.Expression、Purification of CMV 2b Protein and Enhancing Suppressor Activity by Fusion Strategy;CMV 2b蛋白的原核表达纯化及融合策略增强抑制子活性初探
4.antibody fusion protein抗体融合蛋白（质）
5.Adjuvant Activity of the Flagellin of Salmonella typhimurium in the Immune Response of Fusion Protein of Newcastle Disease Virus in Mice沙门菌鞭毛蛋白增强新城疫病毒融合蛋白在小鼠中的免疫原性
6.Prokaryotic Expression and Purification of Fusion Protein of PTD-SH2;PTD-SH2融合蛋白的原核表达和纯化
7.The Purification of sflk1-IFN-γ Fusion Protein;sflk1-IFN-γ融合蛋白的分离与纯化
8.The Construction, Expression and Purification of Recombinant IL-4 Fusion Protein;重组IL-4融合蛋白的构建、表达及纯化
9.Expression of TrxA-EstB1 Fusion Protein and Its Purification, Characterization of Enzyme Activity;TrxA-EstB1融合蛋白的表达、纯化与活性分析
10.Study on the Expression and Purification of GST-p33~(ING1b) Fusion Protein;GST-p33~（ING1b）融合蛋白表达及纯化的研究
11.Expression, Purification, Identification of Fusion Protein PTD-SARAPTD-SARA融合蛋白的表达、纯化及鉴定
12.Expression and Purification of GST-HPV16E7 Fusion ProteinGST-HPV16E7融合蛋白的原核表达与纯化
13.Expression,Purification and Identification of GST-Dot4 Fusion ProteinGST-Dot4融合蛋白的表达·纯化及鉴定
14.Purification of Fusion Protein EGFRi-IL-24 and Bio-Activity Assay融合蛋白EGFRi-IL-24的纯化及活性鉴定
15.Expression and Purification of Fusion Protein SUMO-BMP7SUMO-BMP7融合蛋白的表达及纯化
16.The Human OX40 Fusion Proteins Expression and Purification人OX40融合蛋白的表达及其纯化
17.Fermentation and Purification of the Fusion Protein of Interleukin-2/Human Serum Album人白介素-2/人血清白蛋白融合蛋白的发酵与纯化研究
18.Purification of GLP-1/albumin Expressed in Pichia pastoris;在毕赤酵母菌中表达的hGlp-1-白蛋白融合蛋白的纯化
相关短句/例句
fusion protein purification融合蛋白纯化
3)fusion protein融合蛋白
1.Molecular design and characterization of recombinantfusion protein of human IFNα_(2a)-THYα_1;重组人干扰素α_(2a)和胸腺肽α_1融合蛋白的分子设计及其基因表达产物的鉴定
2.Expression and purification of glycerol dehydratase β-subunit in E.coli asfusion protein;甘油脱水酶β亚基融合蛋白在大肠杆菌中的表达和纯化
3.Pro-apoptotic effect of recombinant anti-HER2fusion protein ScFv/tBid gene on osteosarcoma E10 cells;重组抗HER2融合蛋白基因ScFv/tBid对骨肉瘤E10细胞的促凋亡作用
4)Fusion proteins融合蛋白
1.Separation and purification of recombinant fusion proteins of IFNα_(2b)-THYα_1;重组人干扰素α_(2b)和胸腺肽α_1融合蛋白的分离纯化
2.After induction by isopropyl β-D-1-thiogalactopyranoside(IPTG),the observed yield of crude fusion proteins was up to 260 mg/L.将构建的含有胸腺肽α1融合蛋白的编码基因插入表达质粒pET28a(+)中,并在大肠杆菌BL21中进行表达,该菌经IPTG诱导融合蛋白高表达后,离心收集菌体,超声波破碎,包含体裂解,目标蛋白质的体外变性复性后,经DEAE-Sepharose Fast Flow阴离子交换层析纯化,每升菌液中可获得260 mg融合蛋白。
3.Objective: To express the fusion proteins containing VP3 and Tat-PTD (protein transduction domain) proteins, and then purify and dialysis on this fusion proteins.目的:原核表达VP3与Tat-PTD(protein transduction domain)的融合蛋白,并纯化此融合蛋白,观察Tat-PTD介导VP3进入胃癌细胞SGC7901的透膜效应及VP3在肿瘤细胞中的定位,检测融合蛋白诱导细胞凋亡情况。
5)recombinant protein融合蛋白
1.Objective To explore the reasons for the low intracellular transduction efficiency of a previously constructed His-TAT-Flagrecombinant protein and establish a more efficient transduction system.目的探索本室构建的pET14b-His-TAT-Flag重组载体表达的融合蛋白在细胞中转导效率低的原因,建立高效的细胞内转导系统。
2.The purifiedrecombinant protein was analyzed by SDS-PAGE and Western blotting,the results showed that the CP fragment was highly expressed.重组蛋白经过Ni+-NTA纯化后,SDS-PAGE和Western blotting分析结果表明,TRSV-CP基因在大肠杆菌中获得了高效表达,产生的融合蛋白分子质量约为34 ku,并具有免疫学活性。
3.Arecombinant protein with about 29kD was expressed stably in the form of insoluble inclusion bodies after IPTG induction.PCR扩增拟南芥MnSODcDNA的保守区段,构建pET-SOD重组质粒,转化大肠杆菌JM109穴DE3雪,IPTG诱导融合蛋白高效表达;经检测表达产物占菌体总蛋白的69%,且以不溶的包涵体形式存在;表达产物变性后经Ni-NTASuperflow亲和柱分离纯化,得到相对分子质量约为29000的PAGE纯蛋白。
6)fused protein融合蛋白
1.Expression offused protein A-green fluorescent protein (PA-GFP);蛋白A-绿色荧光蛋白融合蛋白的构建与表达
2.AIM: To construct the prokaryotic recombinant plasmid to express HBV truncated C gene with preS1 gene and to acquire and purify thefused protein for antigenic analysis.目的:构建乙型肝炎病毒(HBV)截短C基因和前S1基因重组原核表达质粒,获得融合蛋白的表达并进行抗原性分析。
3.A method for the purification of expressed maltose binding proteinfused protein by using potato starch instead of crosslinked amylose have been developed.用马铃薯淀粉柱可以直接分离麦芽糖结合蛋白乳酸脱氢酶辅酶结合结构域融合蛋白，并得到满意的结果。
延伸阅读
强化强化reinforcement影像学术语。CT和MR检查中，为提高组织的对比，向血管或体腔内引入人工对比物质后扫描的方法称增强扫描(enhanced scan)，引入人工对比物质后出现的组织的密度或信号变化称强化。不论CT或MR中，引入对比剂后一些正常组织结构可以出现强化，称生理性强化(physiological enhancement)；引入对比剂后，正常时不应出现强化的组织结构出现强化则称病理性强化(pathological enhancement)。
