Volume exclusion chromatography, also known as size-exclusion chromatography, is a powerful technique for separating and analyzing macromolecules based on their size. This chromatographic method utilizes porous beads to separate molecules according to their hydrodynamic size, with larger molecules being eluted earlier than smaller ones. The ability to separate biomolecules such as proteins, polysaccharides, and nucleic acids makes volume exclusion chromatography an indispensable tool in biochemistry and biotechnology. One example of its application is in the purification of proteins from complex mixtures, where the larger impurities are excluded from the pores of the stationary phase, allowing the target proteins to be cleanly separated. This technique is widely used in pharmaceutical development, structural biology, and medical research, owing to its efficiency and versatility in characterizing macromolecular samples.
体积排阻色谱,size-exclusion chromatography
1)size-exclusion chromatography体积排阻色谱
1.Retention data of polystyrene samples of narrow molecular size distribution and known average ~molecular mass from 418 to 190 000 were measured on radial flow monolithic column bysize-exclusion chromatography (SEC).利用体积排阻色谱原理,在自制的径向色谱柱上,测定了具有窄分子量分布且已知平均分子量418～190000的聚苯乙烯标准品的保留数据,这些数据可用于计算柱子的外部(55。
2.Investigations on protein refolding bysize-exclusion chromatography (SEC) was carried out by using lysozyme as a model system.以溶菌酶为模拟体系对体积排阻色谱法进行蛋白质折叠过程实验研究 。
英文短句/例句
1.Renaturation of Reduced/denatured Lysozyme by Size Exclusion Chromatography还原变性的溶菌酶的体积排阻色谱复性
2.Renaturation and Simultaneous Purification of Recombinant Human Granulocyte Macrophage Colonly Stimulating Factor(rhGM-CSF) with Size Exclusive Chromatography;体积排阻色谱法(SEC)对重组人粒细胞-巨噬细胞集落刺激因子的复性并同时纯化
3.Determination of Molecular Weight and Molecular Weight Distribution of Low Molecular Weight Heparin by High Performance Size Exclusion Chromatograghy with Multiangle Laser Light Scatter高效体积排阻色谱与多角激光光散射仪联机测定低分子肝素分子量及分子量分布
4.The Determination of Plasmid Concentration in lysate by Size Exclusion Chromatography体积排阻液相色谱法质粒的含量测定
5.steric exclusion chromatography空间排阻色谱(法)
6.Speciation Analysis of Cadmium in Indian Mustard(Brassica Juncea)by Size Exclusion Chromatography-High Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry体积排阻高效液相色谱-电感耦合等离子体质谱测定印度芥菜中镉的形态
7.gas volumeteric chromatography气体体积色谱(法)
8.exclusion chromatography凝胶色谱法;排除色谱法;排阻色谱法;分子筛色谱法
9.Subtractive Analysis of Size Exclusion Chromatography and Fingerprint for Radix Salviae Miltiorrhizae Injection注射用丹参分子排阻色谱及指纹图谱差减分析
10.Determination of molecular weight averages and molecular weight distribution of polystyrene standards by steric exclusion chromatographyGB/T6599-1986体积排斥色谱法测定聚苯乙烯标准样品的平均分子量及分子量分布
11.Main separation mechanism of high performance liquid chromatography contains absorption, distribution, ion exchange and exclusion function.高效液相色谱法的主要分离机制有吸附、分配、离子交换和排阻作用。
12.Study on relative molecular mass distribution of protein and peptide in donkey-hide glue by gel exclusion chromatography凝胶排阻色谱法研究阿胶中蛋白质及多肽相对分子质量分布规律
13.A Size-exclusion Chromatography with Multiple Angle Laser Light Scattering Study of Molecular Features of Starch高效排阻色谱-多角度激光散射分析淀粉分子特征
14.HPLC determination of acetate in dialysate高效液相离子排阻色谱法测定血液透析液中醋酸根含量
15.HPSEC determination of the polymers in cefotaxime sodium高效分子排阻色谱法分析头孢噻肟钠中的聚合物
16.Studies on the Enantioseparation of Several β-receptor Blocking Drugs by TLC;薄层色谱法拆分几种β-受体阻滞剂药物的研究
17.Chemical reagents--Determination of specific surface area of solid supports used in chromatographyGB/T2922-1982化学试剂色谱载体比表面积的测定方法
18.Cubic content, volume, or displacement.体积，容器，排水量
相关短句/例句
size exclusion chromatography体积排阻色谱
1.The separation effects of alkylphenol ethoxylates in different liquid chromatography modes,such as reversed-phase liquid chromatography,bonded normal-phase liquid chromatography,silica adsorption chromatography andsize exclusion chromatography were investigated.考察了烷基酚聚氧乙烯醚在反相色谱、正相键合色谱、硅胶吸附色谱、体积排阻色谱4种不同液相色谱分离模式中的分离效果,分别采用Kromasil C18(250 mm×4。
2.Renaturation of reduced/denatured Lysozyme in non-oxidation and reduction condition was studied withsize exclusion chromatography used twice.探讨二次体积排阻色谱在非氧化还原平衡体系及中性条件下对还原变性的溶菌酶(Lys)的复性。
3)size exclusion chromatography体积排阻色谱法
1.In order to study the renaturation mechanism of denatured protein in denaturant (solution,) the renaturation and separation process for three kinds of lysozyme molecules, which were (separately) denatured by urea and guanidine hydrochloride in the presence of reducing (agents,) was studied bysize exclusion chromatography.与稀释法复性相比较,用体积排阻色谱法复性时所形成的折叠中间体二分子集聚体的量要远远低于用稀释法所形成的集聚体的量。
2.Objective To determine the molecular weight(Mr) and its distribution of hyaluronic acid(HA) by the combination of multi-angle laser light scattering instrument andsize exclusion chromatography(MALLS-SEC).目的多角度激光光散射仪与体积排阻色谱法联用(MALLS-SEC)测定透明质酸钠(HA)相对分子质量(Mr)及其分布。
4)size-exclusion electrochromato-graphy体积排阻电色谱
5)High-performance size exclusion chromatography高效体积排阻色谱法
6)HPSEC高效体积排阻色谱
1.The molecular weight distribution of hydrolysis was identified by high performance size exclusion chromatography(HPSEC).5%的酪朊酸钠进行酶解,控制酶解时间分别为2、10、48 h,利用高效体积排阻色谱(HPSEC)对不同酶解时间的酶解液进行定性和定量分析,根据分析结果对Neutrase酶解酪朊酸钠的反应过程进行初步推断,所得到的肽分子量分布图谱对该酶解反应具有理论指导意义。
延伸阅读
离子排阻色谱法分子式：CAS号：性质：以唐南平衡为基础的离子交换色谱法。采用强酸或强碱离子交换树脂为固定相，流动相通常是水。用于无机、有机物复杂混合物的分离。
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